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While clinical research findings are more easily extrapolated and integrated to clinical practice, basic research and especially suitable animal models have provided valuable insights to the molecular level and have allowed researchers to manipulate genetic factors in order to study their role. However, conventional animal models that are not so sophisticated, like the modern genetically engineered rodents that develop spontaneous disease, seem to still carry a significant role in studying the pathways that lead to overt Crohn's disease. Among them, hapten reagent 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced colitis introduced in 1989 by Morris et al. bears a pivotal role especially in the pre-clinical testing of various chemical or natural compounds in terms of their anti-inflammatory and/or anti-oxidative effects. Briefly, TNBS colitis belongs to the group of chemically induced colitis animal models that includes among others DSS colitis, and oxazolone colitis , , , . Those three are the most commonly utilized animal models of that category and have shown a significant consistency that reflects to their extensive use during the last decades. 2b1af7f3a8