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iPSC will lead to a breakthrough in regenerative medicine, although several issues for clinical use remain to be solved. Because MSC and EPC have been already applied clinically, we believe our studies using these cells and materials will enable further development of clinical use in the near future. To obtain plenty of CD34+ or CD133+ cells, mobilization from bone marrow (in-vivo expansion) with G-CSF (granulocyte-colony-stimulating factor) and subsequent aphaeresis will be required, because of their low frequency. Therefore, as a future option, ex-vivo expansion of CD34+ or CD133+ cells by use of optimized medium containing growth factor/cytokine cocktails has been tried, to establish a less invasive and less costly approach for EPC-assisted tissue regeneration. This new therapeutic approach will enable more promising and comprehensive tissue repair/regeneration in orthopaedics. The immediate purpose is more effective and less invasive cell therapy, with spatial control of transplanted cells by use of an external magnetic force. Although there have been many reports of the effectiveness of cell therapy in tissue regeneration, its mechanism is yet to be fully elucidated. Analysis of miRNA/exosome in cell therapy has the potential to clarify this mechanism, thus leading to novel alternative cell therapy for tissue regeneration (Fig. 3). Several reports have revealed that therapeutic trials by administration of synthetic miRNA or modified antisense in vivo have been conducted in many fields, hence, miRNA therapy in clinical orthopaedics will be introduced in the near future. It is likely that the combination of miRNA and an external magnetic device will be more useful and safe, so any accompanying challenges should be examined to discover the most effective and least invasive methods.
To summarize, we discover a magnetic photogalvanic effect to generate the photocurrent by breaking the momentum-inversion symmetry in the band structure. This mechanism induces a large photoconductivity in the AFM phase of the bilayer CrI3 despite no electric polarization. The photocurrent appears for the linearly polarized light but vanishes for the circularly polarized one in the 2D system. It exhibits an injection-current-like feature and is proportional to the relaxation time, the resonant transition rate and SOC. Tuning the magnetic structure is a sensitive handle to manipulate the photocurrent. Although the bilayer CrI3 exhibits a record photoconductivity, it seems possible to design even better nonlinear magnetic materials, for example by considering stronger SOC and longer relaxation time. There are plenty of AFM and FM materials, such as magnetoelectric multiferroic compounds56,57, waiting for exploration.
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